Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
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Effects of R-(−)-BPAP on the Expressions of Neurotrophins and Their Receptors in Mesencephalic Slices
Chieko HiramiKazue TakahataSeiichiro ShimazuFumio YonedaKyozo HayashiHiroshi KatsukiAkinori Akaike
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2005 Volume 28 Issue 8 Pages 1524-1526

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Abstract

R-(−)-1-(Benzofuran-2-yl)-2-propylaminopentane [R-(−)-BPAP] enhances electric field stimulation-induced release of catecholamine from isolated brain stem and ameliorates motor deficits in rats. We evaluated the effects of R-(−)-BPAP on the expression of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3), and their receptors, trkB and p75NTR in rat mesencephalic slice cultures. Levels of mRNA and protein were measured at 48 h after R-(−)-BPAP treatment by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. R-(−)-BPAP significantly increased the mRNA and protein levels of BDNF, without affecting the level of NT-3 mRNA. In addition, R-(−)-BPAP significantly increased the mRNA level of trkB, but not that of p75NTR. These effects of R-(−)-BPAP may result in enhanced BDNF/trkB signaling, and could thus underlie the potential neurotrophic and antidepressant actions of this drug.

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© 2005 The Pharmaceutical Society of Japan
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