Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Notes
Development and Application of RAPD-SCAR Marker for Identification of Phyllanthus emblica LINN.
Warude DnyaneshwarChavan PreetiJoshi KalpanaPatwardhan Bhushan
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2006 Volume 29 Issue 11 Pages 2313-2316

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Abstract
Correct genotype identification of medicinal plant material remains important for botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated need for newer methods in quality control of botanicals. The present study was carried out to develop DNA based marker for identification of Phyllanthus emblica LINN. A putative marker (1.1 kb) specific for P. emblica was identified by Random Amplified Polymorphic DNA (RAPD) technique. Sequence Characterized Amplified Region (SCAR) marker was developed from the RAPD amplicon. The SCAR marker was found useful for identification of P. emblica in its commercial samples and Triphalachurna, a multi-component Ayurvedic formulation.
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© 2006 The Pharmaceutical Society of Japan
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