Abstract
The morphology, structure, and antigenicity of the cells and the cell wall mannans of the Candida guilliermondii IFO 10279 strain cultivated at 33 and 34 °C for 48 h in yeast extract-added Sabouraud liquid medium (YSLM) were compared with those cultivated at 27 °C and 33 °C and then at 27 °C (33—27 °C). This strain showed little growth at higher than 35 °C. The density of the yeast formed cells decreased, with dry weights of about 50% at 33 and 34 °C, and only the cells at 34 °C revealed a failure of cytokinesis. The structure of the mannans revealed by 1H-NMR analysis that the mannans obtained at both 33 and 34 °C had drastically decreased two consecutive β-1,2-linked mannopyranose units at the nonreducing terminal of the α-linked oligosaccharides and increased one β-1,2-linked mannopyranose unit at the nonreducing terminal attached to the α-1,3-linked mannose unit and the non-reducing terminal α-1,3- and α-1,2-linked mannopyranose units. The enzyme-linked immunosorbent assay (ELISA) showed that the mannans obtained at 33 and 34 °C had decreased reactivity against the factor serum 9 and increased its reactivity against the factor serum 4, in the commercially available factor serum kit ‘Candida Check’.
