Structural Changes of Cell Wall Mannans of Candida guilliermondii IFO 10279 Strain Cells Cultured at High Temperature

Abstract

The morphology, structure, and antigenicity of the cells and the cell wall mannans of the Candida guilliermondii IFO 10279 strain cultivated at 33 and 34 °C for 48 h in yeast extract-added Sabouraud liquid medium (YSLM) were compared with those cultivated at 27 °C and 33 °C and then at 27 °C (33—27 °C). This strain showed little growth at higher than 35 °C. The density of the yeast formed cells decreased, with dry weights of about 50% at 33 and 34 °C, and only the cells at 34 °C revealed a failure of cytokinesis. The structure of the mannans revealed by ¹H-NMR analysis that the mannans obtained at both 33 and 34 °C had drastically decreased two consecutive β-1,2-linked mannopyranose units at the nonreducing terminal of the α-linked oligosaccharides and increased one β-1,2-linked mannopyranose unit at the nonreducing terminal attached to the α-1,3-linked mannose unit and the non-reducing terminal α-1,3- and α-1,2-linked mannopyranose units. The enzyme-linked immunosorbent assay (ELISA) showed that the mannans obtained at 33 and 34 °C had decreased reactivity against the factor serum 9 and increased its reactivity against the factor serum 4, in the commercially available factor serum kit ‘Candida Check’.