Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Soluble Guanylyl Cyclase Inhibitor Prevents Sema3F-Induced Collapse of Axonal and Dendritic Growth Cones of Dentate Granule Cells
Ryuji X. YamadaNorio MatsukiYuji Ikegaya
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2006 Volume 29 Issue 4 Pages 796-798


Controlling axon and dendrite elongation is critical in developing precise neural circuits. Using isolated cultures of dentate granule neurons, we succeeded in simultaneously monitoring the behaviors of axonal and dendritic outgrowth. Our previous study shows that cAMP contributes differentially to Sema3F-induced responses of axons and dendrites, but we report here that the cGMP modulation does not have such a striking axo-dendritic difference. Treatment with Sema3F induced collapse of about 90% growth cones, and pretreatment with 1 μM LY83583, an inhibitor of soluble guanylyl cyclase, partially alleviated the collapse of both axons and dendrites. Thus, unlike cAMP, cGMP modulates axonal and dendritic extension in a similar manner.

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© 2006 The Pharmaceutical Society of Japan
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