Clinical studies had suggested that there were sex differences in pharmacological and side effects of pioglitazone. However, there are few studies on the sex differences of peroxisome proliferator-activated receptor (PPAR) γ expressions in rat adipose tissues. We investigated the sex differences in peroxisome proliferator-activated receptor (PPAR) γ expressions in rat adipose tissues. Subcutaneous abdominal adipose tissues and perigonadal adipose tissues were obtained from male and female Wistar rats (12 weeks of age). Expressions of PPARγ protein were determined by Western blot analysis with anti-PPARγ antibody. Vaginal smear check was performed in female rats. We obtained adipose tissues from females, according to the different phases of the estrous cycle. Both PPARγ1 and γ2 subtypes were expressed in subcutaneous adipose tissues. Expressions of PPARγ2 in subcutaneous adipose tissues were significantly higher in males than in females. On the other hand, expressions of PPARγ2 in perigonadal adipose tissues were significantly higher in females than in males. Expressions of PPARγ2 in perigonadal adipose tissues in females significantly decreased during diestrus. It can be suggested that the sex hormones might affect the expressions of PPARγ2 in adipose tissues.
2007 The Pharmaceutical Society of Japan