Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
Comparative Study of Circadian Variation in Numbers of Peripheral Blood Cells among Mouse Strains: Unique Feature of C3H/HeN Mice
Naoki OhkuraKatsutaka OishiYuji SekineGen-ichi AtsumiNorio IshidaJuzo MatsudaShuichi Horie
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2007 Volume 30 Issue 6 Pages 1177-1180


We examined strain differences in numbers of blood cells and their circadian rhythms in male Jcl:ICR, BALB/cA, C57BL/6J and C3H/HeN mice. The total numbers of circulating white blood cells (WBCs) were increased during subjective day and night, and the peaks in the active period were common to all strains. However, the number of WBCs in C3H/HeN mice remained lower and plasma levels of corticosterone (CS) were slightly higher throughout the day compared with the other strains. The numbers of circulating red blood cells (RBC) also differed according to strain. The numbers of RBCs, hematocrit (HCT) and hemoglobin (HGB) were considerably lower in C3H/HeN mice compared with the other strains, although mean corpuscular hemoglobin (MCH) and mean corpuscular volume (MCV) were highest among the tested strains. We found that serum erythropoetin (EPO) levels were considerably higher in C3H/HeN mice than in the other three strains. The high EPO level might be related to the unique features of RBCs in C3H/HeN mice. The present observations provide basic information about the numbers of peripheral blood cells and their circadian rhythm in mouse models and also demonstrate a unique feature of C3H/HeN mice.

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© 2007 The Pharmaceutical Society of Japan
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