Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
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Validation of 13C-Acetic Acid Breath Test by Measuring Effects of Loperamide, Morphine, Mosapride, and Itopride on Gastric Emptying in Mice
Kenjiro MatsumotoHiroshi KimuraKimihito TashimaMasayuki UchidaSyunji Horie
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2008 Volume 31 Issue 10 Pages 1917-1922


Several methods are used to evaluate gastric motility in rodents, but they all have technical limitations. Recent technical developments enable a convenient method to evaluate gastric motility. The 13C-acetic acid breath test in rodents is a non-invasive and repeatable method that can be used without physical restraints. The present study aimed to validate the 13C-acetic acid breath test by measuring the effects of loperamide, morphine, mosapride, and itopride on gastric emptying in mice. Loperamide (1—10 mg/kg) and morphine (1.25—10 mg/kg) slowed gastric emptying and decreased the maximum concentration (Cmax) and area under the curve (AUC90 min) value in a dose-dependent manner. Mosapride (0.2—5 mg/kg) accelerated gastric emptying and increased Cmax value. Mosapride (20 mg/kg) did not accelerate gastric emptying on the 13C-breath test. Itopride (30 mg/kg, per os) significantly accelerated gastric emptying compared with the vehicle group. In a comparison with the conventional phenol red test, there was a correlation between the Cmax value of breath test and gastric emptying (%) of phenol red tests in treatment with loperamide or mosapride. These results indicate that the 13C-acetic acid breath test is an accurate, noninvasive, and simple method for monitoring gastric emptying in mice. This method is useful to assess the effect of drugs and gut function pharmacologically.

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© 2008 The Pharmaceutical Society of Japan
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