Quantitation of Biotin-Binding Immunoglobulins G, A, and M in Human Sera Using F(ab′)₂Anti-human Immunoglobulin-Coated Microplates

Abstract

Biotin-binding IgG (B-IgG) in human sera was quantified using previously developed F(ab′)₂anti-human IgG-coated multiwell microplates (Muratsugu M. et al., 2003, Biol. Pharm. Bull., 26, 1605—1608). The levels of B-IgG in sera, however, were higher than those we predicted. In this study, we modified the assay using F(ab′)₂anti-human IgG-coated multiwell microplates and successfully quantified the levels of B-IgG in sera. The cause of the unpredicted results was discussed in the text. In addition, the levels of biotin-binding IgA (B-IgA) and IgM (B-IgM) in sera could be measured using F(ab′)₂anti-human IgA- or IgM-coated multiwell microplates. We quantified B-IgG, B-IgA, and B-IgM in sera from healthy specimens and patients with bronchial asthma, atopic dermatitis, epilepsy, and juvenile rheumatoid arthritis.