Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Inhibitory Effect of the Compounds Isolated from Rhus verniciflua on Aldose Reductase and Advanced Glycation Endproducts
Eun Ha LeeDae-Geun SongJoo Young LeeCheol-Ho PanByung Hun UmSang Hoon Jung
Author information

2008 Volume 31 Issue 8 Pages 1626-1630


The aim of this paper was to evaluate active principles for diabetic complications from Rhus verniciflua. Nine compounds were isolated via bioactivity guided fractionation and isolation and tested for their effects on recombinant human aldose reductase and advanced glycation endproducts. Butein and sulfuretin isolated from ethyl acetate fraction were found to possess strongly both forms of aldose reductase and advanced glycation endproducts inhibition. The inhibitory activity of butein against a recombinant human aldose reductase (IC50 value: 0.5 μM) was 2.6 times more potent that of epalrestat as a positive control (IC50 value: 1.3 μM). The inhibitory potency of sulfuretin (IC50 value: 124.7 μM) on advanced glycation end-products was about 10 times more potent that of aminoguanidine as a positive control (IC50 value: 1231.0 μM). These compounds all displayed antioxidative activity which was measured by Photochem® apparatus. It was concluded, therefore, butein and sulfuretin have antioxidative as well as aldose reductase and advanced glycation endproducts inhibitory effects. As a result, these compounds could be proposed as a leading compound for further study as a new natural products drug that could be used for diabetic complications.

Related papers from these authors
© 2008 The Pharmaceutical Society of Japan
Previous article Next article