Na+–Ca2+ Exchanger Expression and Its Modulation

Junko Kimura; Tomoyuki Ono; Kazuho Sakamoto; Emi Ito; Shinya Watanabe; Sachiko Maeda; Yayoi Shikama; Midori Sasaki Yatabe; Isao Matsuoka

doi:10.1248/bpb.32.325

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Na⁺–Ca²⁺ Exchanger Expression and Its Modulation

Junko Kimura, Tomoyuki Ono, Kazuho Sakamoto, Emi Ito, Shinya Watanabe, Sachiko Maeda, Yayoi Shikama, Midori Sasaki Yatabe, Isao Matsuoka

Author information

Junko Kimura
Department of Pharmacology, Fukushima Medical University, School of Medicine
Tomoyuki Ono
Department of Pharmacology, Fukushima Medical University, School of Medicine
Kazuho Sakamoto
Department of Pharmacology, Fukushima Medical University, School of Medicine
Emi Ito
Department of Clinical Informatics, Tokyo Medical and Dental University School of Medicine
Shinya Watanabe
Department of Clinical Informatics, Tokyo Medical and Dental University School of Medicine
Sachiko Maeda
Department of Pharmacology, Fukushima Medical University, School of Medicine
Yayoi Shikama
Department of Pharmacology, Fukushima Medical University, School of Medicine
Midori Sasaki Yatabe
Department of Pharmacology, Fukushima Medical University, School of Medicine
Isao Matsuoka
Department of Pharmacology, Fukushima Medical University, School of Medicine

Keywords: nicotine, Na⁺–Ca²⁺ exchanger, gene expression, microarray analysis, cardiac myocyte, brain

JOURNAL FREE ACCESS

2009 Volume 32 Issue 3 Pages 325-331

DOI https://doi.org/10.1248/bpb.32.325

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Abstract

Here we reviewed our recent work on the chronic effects of nicotine on the Na⁺–Ca²⁺ exchanger (NCX) gene and protein expressions in various organs of rats treated with nicotine in the drinking water for 4—12 weeks. Microarray analysis and reverse transcriptase-polymerase chain reaction (RT-PCR) did not detect significant changes in NCX mRNA expression in cerebral cortex, hippocampus, heart and skeletal muscle. However, NCX1 protein was up-regulated by nicotine in cerebral cortex and hippocampus, but was down-regulated in the heart. NCX2 protein was up-regulated by nicotine in hippocampus. We suggest that although mRNA change was insignificant, NCX protein expression was altered by chronic nicotine administration in brain and heart in rats. We also reviewed our work on modulators of NCX gene expression and function in cardiac myocytes.

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