Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
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Expression and Secretion of Cholera Toxin B Subunit in Lactobacilli
Takahiro OkunoNobuhiro KashigeTomomitsu SathoKeiichi IrieYukihiro HiramatsuTanjina SharminYuki FukumitsuSaori UyedaSeitaro YamadaTetsuya HarakuniTakeshi MiyataTakeshi ArakawaMasumi ImotoAkihisa TodaYukihiko NakashimaFumio Miake
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2013 Volume 36 Issue 6 Pages 952-958


Lactic acid bacteria (LAB) are used in various fields, including in food and medical supplies. There has been a great deal of research into vaccine development using LAB as carriers due to their “generally recognized as safe” status. Cholera is an infectious disease that causes diarrhea due to cholera toxin (CT) produced by Vibrio cholerae. The pentameric cholera toxin B (CTB) subunit has no toxicity, and is used as an antigen in cholera vaccines and as a delivery molecule in vaccines to various diseases. In this study, we generated recombinant LAB expressing and secreting CTB. Here, we first report that CTB expressed and secreted from LAB bound to GM1 ganglioside. The secreted CTB was purified, and its immunogenicity was determined by intranasal administration into mice. The results of the present study suggested that it may be useful as the basis of a new oral cholera vaccine combining LAB and CTB.

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© 2013 The Pharmaceutical Society of Japan
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