Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
Rapid detection of avian influenza A virus (H7N9) by lateral flow dipstick-recombinase polymerase amplification
Shiwei MaXue LiBo PengWeihua WuXin WangHui LiuLihong YuanShisong FangJiahai Lu
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JOURNALS FREE ACCESS Advance online publication

Article ID: b18-00468

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Abstract

Avian influenza A (H7N9) virus has caused several epidemics and infection in both human and poultry. With mutation, the H7N9 virus gained its fifth endemic in China. Early diagnosis is crucial for the control of viral spread in poultry and prognosis of infected patients. In this study, we developed and evaluated a lateral flow dipstick recombinase polymerase amplification (LFD-RPA) assay for rapid detection of both hemagglutinin and neuraminidase gene of H7N9. Our H7-LFD-RPA and N9-LFD-RPA assay were able to detect 32 fg H7N9 nucleic acid which is more convenient and rapid than previous methods. Through detecting 50 influenza positive samples, cross-reaction was not found with other subtypes of influenza virus. The 100% analytical specificity and sufficient analytical sensitivity results agreed the real time reverse transcription polymerase chain reaction (RT-PCR) assay. The results data demonstrated that our method performed well and could can be applied to the detection of H7N9 virus. This LFD-RPA assay provides a candidate method for rapid point-of-care diagnosis of H7N9.

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© 2018 The Pharmaceutical Society of Japan
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