抄録
Antigenic properties of three new types of estriol 16-glucuronide-bovine serum albumin (BSA) conjugates in which the steroid hapten was linked to a carrier protein through the C-2 or C-4 position have been investigated. All antibodies elicited by immunization with these antigens showed the high affinity (Ka=1.41×109-3.50×109M-) and the excellent specificity to estriol 16-glucuronide. No significant cross-reactivity was observed with other glucuronides, sulfates and free steroids except for estriol 17-glucuronide (0.78-1.34%). These antisera appear to be useful for direct radioimmunoassay of estriol 16-glucuronide in biological fluids without prior hydrolysis and chromatographic purification.
