Biological and Pharmaceutical Bulletin
日本薬学会は,1880年に創立された,我が国では歴史ある学会の一つです.現在,約15,000人の会員を擁しており,毎月3誌の学術誌を刊行しております.英文による学術誌の一つとして「Chemical and Pharmaceutical Bulletin」(Chem. Pharm. Bull.)は, 1953年にPharmaceutical Bulletinとして創刊され,その後Chem. Pharm. Bull. と名称を変え,薬学と健康科学に関する化学分野をカバーしています.二つ目として,「Biological and Pharmaceutical Bulletin」(Biol. Pharm. Bull.) があり,これは1978年に創刊されたJournal Pharmacobio-Dynamicsを起源としており,更に1953年に創刊され,2012年に内容を引き継いだJournal of Health Scienceの後継誌として,薬学と健康科学に関する生物学分野を領域としています.英文と和文両方にて構成される学術誌として,「YAKUGAKU ZASSHI」(薬学雑誌)があり,本学術誌は学会創立の翌年(1881年)に創刊され,最も長い歴史を有しています.薬学雑誌では,和文による原著論文・総説等のほか,臨床薬学領域研究については英文による投稿も受け付けています. 日本薬学会におけるこれら学術誌のスコープは,基礎研究から臨床研究に至る幅広い分野に渡りますが,いずれも薬学・健康科学をベースとしています。3誌に投稿された論文の平均審査期間は,現在,投稿された方へ最初の判定を通知するまでに約1か月ですが,更なる時間短縮を目指しています.3誌ともにJ-STAGEにて無料公開しており,研究成果を世に広める一助となることを期待しております.皆様の研究成果をChem. Pharm. Bull.やBiol. Pharm. Bull.,薬学雑誌へ積極的にご投稿下さいますよう,よろしくお願い申し上げます.

大槻 純男
収録数 11,297本
(更新日 2023/12/09)
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
2022 Journal Impact Factor (JIF)
ジャーナル 査読 フリー HTML 早期公開
Scopus Pubmed
46 巻 (2023) 12 号 p. 1661-1665
Framework-Directed Amino-Acid Insertions Generated over 55-Fold Affinity-Matured Antibody Fragments That Enabled Sensitive Luminescent Immunoassays of Cortisol もっと読む

Genetic engineering now enables generation of artificially modified antibodies having higher diagnostic utilities. The authors developed single-chain Fv fragments (scFvs) against cortisol with >55-fold improved affinity (Ka, 2.0-2.2 ´ 1010 M-1) by inserting additional amino acid(s) site-directedly into the framework region 1 of the VH domain. These scFvs were fused with NanoLuc luciferase for the use in an enzyme-linked immunosorbent assay (ELISA) system. The resulting luminescent ELISAs generated dose-response curves with >150-fold higher sensitivity than the colorimetric ELISAs using the scFv without insertion and >8,000-fold higher sensitivity than the ELISA using the mouse antibody from which the scFvs were derived.

46 巻 (2023) 12 号 p. 1676-1682
Method for Preparing Recombinant Galectin-2 Protein without Escherichia coli-Specific Post-translational Modifications もっと読む

E. coli is often employed for the cost-effective production of large quantities of recombinant proteins. Conventionally, it is believed that post-translational modifications, including glycosylation, do not transpire during protein expression in E. coli. However, in the course of preparing recombinant galectin-2 protein using E. coli, the authors discovered that phosphogluconoylation of Lys residues and mistranslation of termination codons occurred. The authors have elucidated strategies to mitigate these occurrences, proposing the addition of tags, substitution of Lys residues, and modification of termination codons. These methods offer valuable means to prevent undesired modifications, ensuring the production of homogeneous recombinant proteins in E. coli.

46 巻 (2023) 12 号 p. 1720-1730
Exploring Cell-Penetrating Peptides as Penetration Enhancers in Eye Drop Formulations Using a Reconstructed Human Corneal Epithelial Model もっと読む

The authors focused on cell-penetrating peptides (CPPs) as penetration enhancers for ocular drug delivery. This study suggested that the CPPs evaluated in this study can be penetration enhancers based on in vitro intracellular uptake using a reconstructed human corneal epithelial model. The CPPs could enhance the penetration of drug molecules into the cornea in cases of coexistence as well as conjugation between CPPs and drug molecules. The result of surface plasmon resonance showed that the electrostatic interaction plays an important role. The authors expect that this fundamental information in this article will support the development of new penetration enhancers in eye drop formulations for ocular drug delivery.

46 巻 (2023) 12 号 p. 1753-1760
Arid5a/IL-6/PAI-1 Signaling Is Involved in the Pathogenesis of Lipopolysaccharide-Induced Kidney Injury もっと読む

Inflammation is responsible for the development of various kidney diseases. Plasminogen activator inhibitor-1 (PAI-1) is involved in the pathogenesis of inflammatory kidney injury; however, the regulatory mechanism of PAI-1 in injured kidneys remains unclear. The authors found that PAI-1 expression was increased in endothelial cells after lipopolysaccharide (LPS, an inflammation inducer) treatment, and pharmacological inhibition of PAI-1 reduced LPS-induced kidney injury. Moreover, IL-6 exacerbated kidney injury concomitant with increased PAI-1 expression, and Arid5a deficiency partially suppressed the expression of IL-6 and PAI-1 in the kidneys after LPS treatment. These findings indicate that the Arid5a/IL-6/PAI-1 signaling is involved in LPS-induced kidney injury.

46 巻 (2023) 12 号 p. 1778-1786
Identification of the Acidification Mechanism of the Optimal pH for RNase He1 もっと読む

Hericium erinaceus secretes an acidic ribonuclease (RNase) He1 belonged to RNase T1 family. The authors decided on the structure of He1 apo form and He1/guanosine complex. The mechanism of acidification of optimal pH in He1 was, in neutral environment, to form the hydrogen bond between Asp 31 on α1β3- loop and His 34 (catalytic residue), and repulsive each other Glu 92 and Asp 93 on β6,7- loop. Structure comparison of He1 with other acidic RNases, Ms and U2, suggested that the acidic residues on α1β3- and β6,7- loop may contribute to the acidification of optimal pH in Ms and U2.

月間アクセス数ランキング (2023年11月)
  • Announcement of Academic Journals’ Awards Biological and Pharmaceutical Bulletin (BPB)
  • Biol. Pharm. Bull. Vol. 46 No. 2
    Current Topics: Cutting Edge Developments in RNA Biology for the Control of Gene Expression