抄録
A previously reported fluorometric high performance liquid chromatographic procedure for separation of 9-anthryldiazomethane (ADAM) derivatives of prostaglandins (PG's) was applied to examine catabolic products of PG's, such as 15-keto-PGE2, 13, 14-dihydro-15-keto-PGE2, 15-keto-PGF2α, and 13, 14-dihydro-15-keto-PGF2α. ADAM derivatives of these metabolites were separated in 30 min on an ODS column with 65% acetonitrile. By this method catabolism of PGE2 and PGF2α in kidney homogenates of rats and rabbits was analyzed. Homogenates of medulla and cortex of rat kidney catabolized PGE2 and PGF2α to 15-keto-PG's. Whereas the homogenate of rabbit kidney cortex yielded 13, 14-dihydro-15-keto-PGE2, that of medulla did not. The conversion of PGF2α to PGE2 was shown to occur in homogenates of rabbit kidney cortex as well as rat kidney medulla and cortex.
