ENHANCEMENT OF SULFANILAMIDE N⁴-HYDROXYLASE ACTIVITY IN KIDNEY AND LIVER MICROSOMES OF RATS BY PRETREATMENT WITH 3-METHYLCHOLANTHRENE TYPE-POLY-CHLORINATED BIPHENYL

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N⁴-Hydroxylation of sulfanilamide was studied with kidney and liver microsomes of rats by means of high performance liquid chromatography. In kidney microsomes, both contents of cytochrome P-450 (448) and activity of N⁴-hydroxylase were markedly increased by pretreatment with 3, 4, 5, 3', 4'-pentachlorobiphenyl (PenCB) (about 4 times), although neither one was increased by 3-methylcholanthrenen (3-MC) and phenobarbital (PB) pretreatments. In liver microsomes, on the other hand, both N⁴-hydroxylase activity and cytochrome P-450 (448) contents were increased by either PenCB or 3-MC pretreatment, whereas by PB pretreatment, N⁴-hydroxylase activity was not changed although cytochrome P-450 contents was increased 2-fold.