Abstract
The physicochemical process whereby bilayer membranes are formed in the chenodeoxycholate, phosphatidylcholine and cholesterol solution is described. Observations were made by electron microscopy using both the negative stain method and the freeze fracture method after excess amounts of cholesterol monohydrate crystals were incubated at varying ratios in sodium taurochenodeoxycholate and phosphatidylcholine solutions. A hypothesis of the transformational processes from bilayer disk to multilamellar liposome in the artificial bile is presented.
