MOLECULAR CLONING OF THE nemA GENE ENCODING N-ETHYLMALEIMIDE REDUCTASE FROM Escherichia coli

Koichi MIURA; Yoshihisa TOMIOKA; Hideaki SUZUKI; Matoko YONEZAWA; Takanori HISHINUMA; Michinao MIZUGAKI

doi:10.1248/bpb.20.110

Koichi MIURA, Yoshihisa TOMIOKA, Hideaki SUZUKI, Matoko YONEZAWA, Takanori HISHINUMA, Michinao MIZUGAKI

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Keywords: Escherichia coli, N-ethylmaleimide reductace, unsaturated fatty acid, Old Yellow Enzyme

JOURNAL FREE ACCESS

1997 Volume 20 Issue 1 Pages 110-112

DOI https://doi.org/10.1248/bpb.20.110

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Abstract

Using the gene mapping membrane technique, we identified a gene (nemA) that encodes N-ethylmaleimide reductace in Escherichia coli. The open reading frame encodes a polypeptide of 365 amino acids with a molecular mass of 39, 514 Da. The deduced amino acid sequence showed a high degree of homology (87% identical) with the pentaerythritol tetranitrate reductase of Enterobacter cloacae and the morphinone reductase of Pseudomonas putida (52% identical).

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