Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
Phenobarbital Molecularly Imprinted Polymer Selectively Binds Phenobarbital
Yoshihisa TOMIOKAYoshiki KUDOTetsuro HAYASHIHitoshi NAKAMURAMasahiro NIIZEKITakanori HISHINUMAMichinao MIZUGAKI
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Volume 20 (1997) Issue 4 Pages 397-400

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Abstract

Molecularly imprinted polymer (MIP) was propared against phenobarbital using methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross linking monomer. We analyzed the recognition properties of the phenobarbital MIP. In some organic solvents, imprinted polymer showed selective binding to phenobarbital. Two dissociation constants of binding were calculated by Scatchard plot analyses; Kd values were 1.8, 121.7 μM, and the number of binding sites was 8.3, 92.3 μmol/g MIP in toluene-heptane-acetic acid (25 : 75 : 1, v/v), respectively. The relationship between the binding affinity to phenobarbital MIP and the polarity of the solvent system, as well as the structure of the template molecule is also discussed.

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