Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
LONG-TERM ADMINISTRATION OF "SHO-SAIKO-TO" INCREASES CYTOCHROME P-450 mRNA LEVEL IN MOUSE LIVER
keisuke KOJIMAHajime MIZUKAMITakako TAZAWAMitsuhiko NOSEMakoto INOUEYukio OGIHARA
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1998 Volume 21 Issue 4 Pages 426-428

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Abstract

Simplified differential display of mRNA was applied to isolate and identify genes transcriptionally regulated in mouse liver by sho-saiko-to administration. A cDNA fragment up-regulated by sho-saiko-to was isolated and characterized. cDNA sequencing and subsequent database analysis revealed that the fragment showed significant sequence similarity with mouse testosterone 16-alpha-hydroxylase (cytochrome P-45016α) cDNA. The increased level of mRNA expression of cytochrome P-45016α in association with sho-saiko-to administration suggests the molecular mechanism of the chemopreventive effect of sho-saiko-to. This result indicates the usefulness of the mRNA differential display technique to investigate the molecular mechanism of Kampo medicine.

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