Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
A Protease Sensitive Region of Plant and Animal Ribonucleases Belonging to the RNase T2 Family
Masanori IWAMAAyumu KUSANOYuko OGAWAKazuko OHGIMasachika IRIE
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1998 Volume 21 Issue 6 Pages 634-637

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Abstract

Recombinant RNase LE from tomato and squid liver RNase Tp, typical plant/animal type RNases belonging to the RNase T2 family, were subjected to limited digestion with several proteases, and the cleavage sites were analyzed by Edman degradation. Recombinant RNase LE was cleaved specifically at the 24th Lys by lysylen-dopeptidase and trypsin, and RNase Tp was cleaved at the 21st Glu by V8 protease. These cleavage sites are located very close to those where the cleavage during preparation of several animal RNase T2 family enzymes was observed. From this finding, it was concluded that the short segment around the 20th amino acid residue in plant/animal RNases in located on the surface of the molecules and forms loops, and is thus very sensitive to proteases.

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