In Vitro Inducible Nitric Oxide Synthesis Inhibitors from Alismatis Rhizoma

Na-Young KIM; Tai-Hyun KANG; Hyun-Ock PAE; Byung-Min CHOI; Hun-Taeg CHUNG; Seung-Woon MYUNG; Yun-Seon SONG; Dong-Hwan SHON; Youn-Chul KIM

doi:10.1248/bpb.22.1147

Na-Young KIM, Tai-Hyun KANG, Hyun-Ock PAE, Byung-Min CHOI, Hun-Taeg CHUNG, Seung-Woon MYUNG, Yun-Seon SONG, Dong-Hwan SHON, Youn-Chul KIM

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Keywords: Alismatis Rhizoma, inducible nitric oxide synthase (iNOS), inhibitor, RAW 264.7 cell, alismol, alisol B monoacetate

JOURNAL FREE ACCESS

1999 Volume 22 Issue 10 Pages 1147-1149

DOI https://doi.org/10.1248/bpb.22.1147

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Abstract

Bioassay-guided fractionation of an aqueous extract of Alismatis Rhizoma has furnished two inducible nitric oxide synthase (iNOS) inhibitory compounds, alismol (1) and alisol B monoacetate (2), together with an inactive triterpene, alisol C monoacetate (3). Compounds 1 and 2 inhibited nitric oxide (NO) synthesis in a dose-dependent manner in murine macrophage-like RAW 264.7 cells stimulated with interferon-γ (IFN-γ) plus lipopolysaccharide (LPS). The inhibitory effects of 1 and 2 on NO synthesis were partly due to suppression of iNOS mRNA expression as determined by Northern blotting.

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