Studies of the Active Substances in Herbs Used for Hair Treatment. II.Isolation of Hair Regrowth Substances, Acetosyringone and Polyporusterone A and B, from Polyporus umbellatus Fries

Abstract

Fractionation of the 50% ethanol extract of Polyporus umbellatus Fries by column chromatography on Amberlite XAD-2, silica gel, Sephadex LH-20 and octadecyl silica gel (ODS) (C18) monitored by a hair-regrowth activity assay, afforded three active principles, 1, 2 and 3. The structures of 1, 2 and 3 were determined as acetosyringone, polyporusterone A, and polyporusterone B by comparison of their spectral data with that of authentic samples, respectively. The effects of several compounds related to acetosyringone, 3, 4-dihydroxybenzaldehyde or polyporusterone A on hair regrowth were also investigated.