Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
Constituents in Watercress : Inhibitors of Histamine Release from RBL-2H3 Cells Induced by Antigen Stimulation
Yukihiro GODAKaori HOSHINOHiroshi AKIYAMATomoko ISHIKAWAYukiko ABETakatoshi NAKAMURAHideaki OTSUKAYoshio TAKEDAAkio TANIMURAMasataka TOYODA
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Volume 22 (1999) Issue 12 Pages 1319-1326

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Abstract

Histamine release inhibitors in watercress (Nasturtium officinale) were isolated using a monitoring system with antigen-stimulated RBL-2H3 cells. Of the 15 compounds isolated, flavonols and megastigmanes significantly inhibited histamine release. Two flavonols, 3-O-sophorosides of rhamnetin and rhamnazin, were new compounds. To investigate the inhibitory mechanism, the effects of rhamnetin, rhamnetin 3-O-sophoroside and an isolated megatigmane glucoside on the increase in the intracellular free calcium concentration were examined at a concentration providing 60% inhibition of histamine release. The results suggest that these compounds did not affect the calcium influx at that concentration. The structure-activity relationships of the megastigmanes on histamine release were also investigated.

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© The Pharmaceutical Society of Japan
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