Separation of Phosphatidylinositol 4-Phosphate from a Mixture with Phosphatidylserine and Phosphatidylinositol by Sep-Pak C₁₈ Cartridge

Abstract

A simple and efficient method for the separation of phosphatidylinositol 4-phosphate (PI 4-P) from phosphatidylinositol (PI) and phosphatidylserine (PS) is described. A mixture of PI, PI 4-P and PS was injected onto a Sep-Pak C₁₈ cartridge. PI and PS were flushed through the cartridge with solvent 1 [methanol-chloroform (3 : 1)] while PI 4-P remained in it. Then the cartridge was inverted, and PI 4-P was eluted backward with solvent 2 [chloroform-methanol-0.5 M aqueous ammonium hydroxide (9 : 7 : 2)].