抄録
Co-occurrence of zinc (Zn) deficiency and iron (Fe) deficiency is common in the world. However, investigations on simultaneous Zn and Fe deficiencies using experimental animals are limited, because of technical difficulties. We established Zn/Fe-deficient animal (rat) model in the previous study. We investigated effects of simultaneous Zn and Fe deficiency on plasma lipids and protein concentrations and enzyme activities. Forty 4-week-old male Sprague-Dawley rats were assigned into 4 dietary treatment groups of 10 each for the 4-week study: Fe-deficient group (FD), Zn-deficient group (ZD), Fe/Zn-deficient group (FZD), and control group (Control). The data were analyzed by SYSTAT ver. 10.2 and Statcel ver.2. The difference between groups was statistically tested by Tukey's simultaneous multiple comparison test and two-way ANOVA. P value less than 0.05 was considered significant. Plasma phospholipids were significantly affected by dietary iron level. Plasma phospholipids of FD and FZD were significantly decreased compared with ZD. Total protein and globulin concentration in plasma of FD were significantly increased compared with Control. Plasma Albumin/globulin ratio of ZD was significantly increased compared with FD. Plasma AST activity was significantly increased by iron deficiency, and plasma ALT activity was significantly increased by zinc deficiency. Plasma ACE activity of FD, ZD and FZD were significantly increased compared with Control.
