2016 年 27 巻 1 号 p. 15-22
Objective: The aim of this study is to establish a simple sample preparation method for analysis of fluoride in biological materials with headspace gas chromatography-mass spectrometer (HS GC-MS).Methods: Urine samples were introduced into headspace glass vials with hydrochloric acid, trimethylchlorosilane and acetaldehyde as the internal standard. Trimethylfluorosilane (TMFS), a derivatized fluoride, was analyzed with HS GC-MS.Results: The calibration curve of TMFS was linear in the investigated range from 0.01 to 15 µg/mL. TMFS showed a constant peak area at pH lower than 3.Conclusion: A simple sample preparation and selective analytical method was established for analysis of fluoride in human urine. This method can be carried out without extraction and reduces solvent consumption.
