抄録
Twelve strains of gram negative, hemophilic, small rod were isolated from gingival sulcus of 14 patients with periodontal disease. These strains were nonmotile and did not form endospores. Capsules have not been demonstrated. All strains gave negative indol, H2S, urease, catalase, oxidase and Voges-Proskauer reactions, all failed to liquefy gelatin and all reduced nitrate. The organism was a strict parasite, growing only in the presence of either hemolyzed serum or washed red cells, and or whole blood, among which the presence of whole blood resulted in the most luxuriant growth. These results suggested that the isolated strains might be included in genus Haemophilus. But the growth occurred not only on the Casman’s basal medium containing both X and V factors, but also on that containing either X or V factor alone. And it could grow on Casman’s basal medium only when both red-cell stromata and serum were added to the medium. It appears that the isolated oral Haemophilus is a distinct species that can be separated from the other members of the genus Haemophilus as described in the seventh edition of Bergey’s Manual of Determinative Bacteriology and from H. vaginalis, to a certain extent, by growth and nutritional characteristics, biochemical activities, pathogenicity for laboratory animals, and the others. It was suggested that the oral Haemophilus, accompanied with the other anaerobic oral organisms, may play some role in the pathogenesis of periodontal disease, because the organism could be isolated only the patients with periodontal disease and their endotoxic activities are as high as oral Veillonella.
