抄録
Paramagnetic profile of hepatic subcellular particles was observed with electron spin resonance (ESR) spectroscopy. ESR signals of mitochondria and microsome revealed a special feature respectively, that is, the former consists of spectrum of non-heme-iron and the later of one of heme-iron (low spin type). These signals were responsible to various reagents, especially reductant and oxidant. Moreover, it was noted that the signal of mitochondria was degraded completely by urea treatment though the microsomal signal remained unchanged. This fact suggests that the non-heme-iron of mitochondria consists of iron protein. The absorption spectra of liver homogenate were identified being composed of the signals of mitochondria and microsome. Basing on these results, ESR spectra of rat hepatoma cells with different strains and life time were detected. A specific three-peaks signal at g=2.00 was observed in the most cells of hepatoma and its structure was discussed.
