2009 年 58 巻 11 号 p. 941-944
A flow-injection amperometric analysis system for the determination of glucose in human urine was analyzed. The glucose sample was mixed with glucose oxidase, and was introduced into the flow-injection system. Hydrogen peroxide produced by an enzymatic reaction was amperometrically detected by a platinum electrode modified with Nafion, and an electrochemically polymerized saccharide substituted polypyrrole. The influence of electroactive compounds such as ascorbic acid, uric acid, and acetaminophen to the glucose response observed in this system was negligible. A good linear relationship between the glucose concentration and the response current was obtained within a concentration of up to 22.4 mmol dm−3. The relative standard deviation for ten replicate analyses of 5.6 mmol dm−3 glucose in human urine was 7.4%.