アナリティカルレポート
固相抽出—液体クロマトグラフィー/質量分析法による環境水中の食品添加物青色1号の定量
2012 年 61 巻 2 号 p. 127-131
詳細
2012 年 61 巻 2 号 p. 127-131
An analytical method for the determination of Brilliant Blue FCF in water by liquid chromatography mass spectrometry (LC/MS) has been developed. A hundred milliliter of water sample portion of passed through an anion exchange solid phase extraction cartridge at a flow rate of 3 mL min−1. The cartridge eluted with 5 mL of 5%-NH4OH/methanol. The eluate was concentrated to ca. 0.5 mL, and Fast Green FCF was added as an internal standard and adjusted to 1 mL with purified water. This solution was determined by LC/MS (ESI negative ion mode). The averages of recoveries of Brilliant Blue FCF from purified water and pond water were 97.4% and 94.8%, respectively. For the 32 rivers in Ibaraki Prefecture investigated by this method, Brilliant Blue FCF was detected from 26 rivers. A detected mean concentration was 0.13 μg L−1, and the maximum concentration was 1.2 μg L−1. This result shows that Brilliant Blue FCF exists in the environment widely.
