法科学的検査のための植物DNA抽出キットの比較

抄録

Recently, many studies have attempted to discriminate and identify forensic botanical samples by DNA analysis. In this study, DNA was extracted from 4 plant species by 4 commercially available kits. Also, whether the purified DNA could provide sufficient quality for PCR amplification was examined. PCR of the rbcL partial region sequence, which is used as markers for phylogenetic and taxonomic studies, was performed after DNA extraction. Amplicon was observed from all of the samples extracted by DNeasy Plant Mini Kit, but not all samples by ISOPLANT and ISOPLANT II, and none of the samples by Genomic-tip. These results suggested that the amounts of the extracted DNA or PCR inhibitor differed depending on sample species and extraction kits. In addition, DNA from different parts of the plant body, such as flower, stem, root and seed, could be extracted using DNeasy Plant Mini Kit, and the rbcL partial region was successfully amplified by PCR. Therefore, the DNeasy Plant Mini Kit was considered to be suitable for DNA analysis of plant samples.