COLOR REACTION BETWEEN PYROGALLOL RED-MOLYBDENUM(VI) COMPLEX AND PROTEIN APPLICATION OF XANTHENE DERIVATIVES FOR ANALYTICAL CHEMISTRY. Part XXXIV

Yoshikazu FUJITA; Itsuo MORI; Shoko KITANO

doi:10.2116/bunsekikagaku.32.12_E379

COLOR REACTION BETWEEN PYROGALLOL RED-MOLYBDENUM(VI) COMPLEX AND PROTEIN

APPLICATION OF XANTHENE DERIVATIVES FOR ANALYTICAL CHEMISTRY. Part XXXIV

Yoshikazu FUJITA, Itsuo MORI, Shoko KITANO

著者情報

キーワード: determination of protein, albumin, spectrophotometry with pyrogallol redmolybdenum(VI) complex, detection test on spot plate, in the presence of gum arabic, urinary protein

ジャーナルフリー

1983 年 32 巻 12 号 p. E379-E386

DOI https://doi.org/10.2116/bunsekikagaku.32.12_E379

詳細

抄録

A color reaction between pyrogallol red(PR) -molybdenum(VI) complex and protein was investigated. A spectrophotometric method using the PR-molybdenum(VI) complex could be used in the concentration range of 0 - 400 μg/10 ml of albumin, where the Sandell sensitivity was calculated to be 0.0048 μg/cm² for albumin at 600 nm. A detection test of protein on a spot plate was also studied. The proposed method was applied to the determination of urinary protein; analytical results were satisfactory.

責任著者(Corresponding author)

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