原子吸光分析法によるα-アミノ酸の間接定量

抄録

α-Amino acids were indirectly determined with good precision by atomic absorption spectrophotometry (A.A.S.). Reagent: To 40ml of 70.75mg of Na₃PO₄·12H₂0/ml solution is added 20ml of 27.79mg of CuCl₂·2H₂O/ml solution with stirring and then centrifuged at 3000rpm. The supernatant is flowed out by decantation. To the precipitate 60ml of 19.04mg of Na₂B₄O₇·10H₂O/ml solution is added and centrifuged at 3000rpm. And the supernatant is flowed out by decantation. This procedure is repeated twice. To the precipitate 3.01g of NaCl is added and diluted to 100ml with 19.04mg of Na₂B₄O₇·10H₂O/ml solution. Procedure: The aqueous sample solution, containing (25500)μg of α-amino acids, is mixed with 1ml of reagent (diluted to ten times), and then brought to 10ml with water. The mixture is shaken for 30s and standed for 2min. After filtration, the copper content in the filtrate is measured by A.A.S. No detectable interference for determination of α-amino acids with any of the other compounds such as acetaldehyde, ethylamine, sodium acetate, glucose, urea, ammonium nitrate, and sodium phosphate, however, Fe³⁺ interfered with the determination of α-amino acids.