高速液体クロマトグラフィーによるヒト尿中遊離型グルココルチコイドの蛍光定量

抄録

In order to determine human urinary cortisol by high performance liquid chromatography and fluorometry, prepurification of cortisol in human urine by an open column chromatography on Amberlite CG-50 was examined. A fraction containing cortisol and cortisone was obtained from human urine by adsorption on a column of Amberlite CG-50 in the presence of 35 % ammonium sulfate at pH 2.5 and elution with acidic aqueous ethanol. Ethyl acetate extract of the fraction was analyzed using ODS column (Finepak C₁₈) and polystyrene gel column (Shodex RS_pak DS-613). Both gave two peaks that had retention time corresponding to that of cortisol and cortisone respectively. The recovery of 1 μg of cortisol and cortisone added to a urine sample was 90 per cent with R. S. D. of 7.0 %(n=5) and 2 ng of cortisol and cortisone could be detected.