COMPARISON OF RETENTION TIMES OF POLYPEPTIDES IN REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY ON POLYSTYRENE RESIN AND ON ALKYL BONDED SILICA

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Retention times were estimated for eleven-tryptic peptides of calmodulin, calmodulin and S100 protein on the various reversed phase under the same elution condition, that is, linear gradient system from 20 % (v/v) to 75 % acetonitrile in 0.1 % (v/v) trifluoroacetic acid. The comparison of retention times was performed on the various combinations of the columns: Polystyrene (Hitachi-Gel 3013-O) vs. C₁₈ silica (Develosil ODS-3), hydroxylmethyl (HO-CH₂-) polystyrene (Hitachi-Gel 3013-O) vs. C₁₈ silica (Develosil ODS-3), C₁ silica (TSK gel TMS-250) vs. C₁₈ silica (Develosil ODS-3) and C₁₈ silica (μ Bondapak) vs. C₁₈ silica (Develosil ODS-3). The relationships in above combination were linear respectively except for hydroxylmethyl polystyrene.