試作キャピラリーオンカラムガスクロマトグラフの性能

抄録

The capillary on-column gas chromatograph presented in the previous paper has three devices mainly designed for (1) column cooling upon sample loading, (2) loading the sample under a condition maintaining flow rate of carrier gas in the column, and (3) cutting the tailing of solvent peak. A method for (1) consists of pulling out the column head from the oven during the sample loading. In this method, the forefront of the sample in the column is already heated and developed while the residual sample is still being injected. The purpose of this paper is to clarify the effect of this process on the performances of the unit in comparison with conventional split methods. The apparatus used for the experiments was newly equipped with a flow control unit designed for (2). The samples were hexane solutions of C₁₀C₃₀ alkanes. Measurements of retention time and peak area were carried out by both on-column and split modes. Any peak with abnormal shape was not observed. Reproducibility of retention time for alkanes by the on-column mode was equal to or better than that by the split mode. In the quantitative analysis, the on-column mode was much better than the split mode in the reproducibility and accuracy.