PART II. ELECTROPHORETICAL STUDIES ON FRACTIONATED GASTRIC JUICE

PERTAINING TO HISTAMINE REFRACTORY ANACIDIC SPECIMEN

Abstract

In comparison to the histamine refractory anacidic specimen of several gastric disorders with acidic control and with carcinomatous specimen, the following conclusions were obtained by electrophoretical investigation in both native gastric juice and in fractionated specimens according to the method as reported by Glass and Boyd.
1. The electrophoretic pattern in native gastric juice of histamine refractory anacidics was placed between that of healthy control and carcinomatous gastric juice. That is to say, the electrophoretic pattern of healthy acidic control shows B₁ and B₄ peak clearly, as compared with B₂ or B₃ peak. The pattern of carcinomatous gastric juice differs from the control pattern in two points, showing that an increase of B₂ and B₃ peak and diminution of B₁ peak were present.
On the other hand, in the histamine refractory anacidics, increase of B₂ and B₃ peak is seen as in the case of carcinomatous specimen, whereas B₁ peak is clearly observed as in the case of the control.
2. In the fractionated specimen, FVIII (MP) is closely connected with B₁ peak (at pH 8.6) or a- and b- peak (at pH 4.5) electrophoretically. FVII (Ms) is connected with B₄ peak (at pH 8.6).
B₂ and B₃ peak, which increased in both histamine refractory anacidics and cancerous specimen, seemed to be connected with FVI and FVII. It is likely that the increase in FV, VI and VII corresponds with the above pathological patterns.
Moreover, it was ascertained that the both fractions of MP (FVIII) and Ms (FVII) in pathological gastric juice consisted of heterogenous components electrophoretically.
3. A considerable part of the constituents in gastric juice with the exception of MP is contained in FV, which is a supernatant of acetone-soluble fraction. And these constituents are transferred largely into FV in the case of pathological gastric juices, i. e., histamine refractory anacidics and carcinomatous gastric juices. Accordingly, it is postulated that acetone-soluble fraction can not be neglected in the pathological gastric juices.