Abstract
1. Polarographical waves and biochemical contents of orcinol reactive, diphenylamine reactive substance, glucosamine, glucuronic acid, tyrosine and of nitrogen were measured on fractionated specimens by means of continuous electrophoresis with regards to three groups of gastric juices, the healthy acidic control, the gastric carcinoma and the histamine refractory anacidic sample.
2. Electrophoretic patterns obtained by continuous electrophoresis agree with that of strip paper electrophoresis. Thus, it was ascertained that the above chemical analyses were performed on electrophoretically homogenous specimens.
3. In the acidic control, the highest polarographical filtrate wave and the highest contents of orcinol chromogen, diphenylamine chromogen, and of tyrosine were observed in B1 peak with the fastest mobility. Hexosamine content was highest in B4, and was also found to exist in the area between B1 and B2. The contents of hexuronic acid and nitrogen were noticed in B4 with slow mobility.
4. In both histamine refractory anacidics and carcinomatous gastric juice, it was found that the above chemical contents observed in B1 were transferred into B2 or B3. And it was of interest to note that the histamine refractory anacidic sample was placed between the acidic control and the carcinomatous sample with respect to their electrophoretic patterns and characteristic behaviours of chemical constituents. Thus the clarification of functional precancerous stage may be expected by investigating chemical constituents, in detail, which are related to B2 and B3.
