PAPER ELECTROPHORETIC STUDIES ON ENZYMES IN THE LIVER OF RATS FED 4-DIMETHYLAMINOAZOBENZENE

I. HISTIDASE

Abstract

1) The activity of histidase in the liver of rats fed 4-dimethylaminoazobenzene (DAB) was studied rather qualitatively by means of paper electrophoresis. The activity was represented in a pattern in consideration of the length of migration of enzyme on the paper.
2). Normal rat liver showed one prominent peak on the activity pattern at the electromigrated location, which reached nearly to an end of the zone of tissue protein. Regenerating liver showed also a similar pattern to that of normal liver.
3) The liver of DAB fed rats in their early experimental days, the cirrhotic liver and hepatoma of prolonged DAB fed rats showed low activity pattern, especially low in hepatoma.
4) Qualitative changes of histidase have not been detected in the course of hepatocarcinogenesis, although the activity diminished keeping step with the progress of pathological changes of the liver.