Abstract
1) Acid and alkaline phosphatase in the homogenate of rats fed 4-dimethylaminoazobenzene (DAB) were investigated by means of paper electrophoresis and the activity was represented in a pattern, details of which have been described.
2) Pattern of acid phosphatase in normal liver showed two prominent peaks at the initial point and at migrated zone of protein respectively. Alkaline phosphatase showed a low maximum at the initial point and a very low curve situated at the migrated part.
3) Patterns of both phosphatases in cirrhotic liver were similar to those of normal liver, but there was a higher peak at the initial point, especially in the case of alkaline phosphatase.
4) In case of hepatoma, pattern of alkaline phosphatase showed a peak of high level at the initial point, higher than that of normal liver and even of cirrhotic liver. The peak of acid phosphatase at this site was lower than that of normal and cirrhotic liver.
5) Patterns of both phosphatases in the livers of DAB fed rats in their fourth week were alike to those of hepatoma.
6) Patterns of alkaline phosphatase in sera of DAB fed rats showed non-detectable differences from that of normal rat sera. A marked peak of bell-shaped curve was observed in α2-globulin fraction and lower one at the starting point.
