Abstract
Conditions for the quantitative determination of ubiquinone in normal rat liver, hepatomas, and fibrosarcomaw ere investigated. For the crude extract of ubiquinone with petroleum ether, the colorimetric assay was found to be appropriate. For the purified ubiquinone fraction from alumina column chromatography, colorimetric and spectrophotometric assays gave satisfactory results.
The ubiquinone content of hepatoma and sarcoma was one-third to one-tenth of that of the normal rat liver. Ubiquinone concentration in mitochondria of sarcoma, on a per mg nitrogen basis, also showed a lower value than that of the normal liver. Hepatoma AH-49 and sarcoma contain UQ9 as the sole ubiquinone.
