CATALASE OF TUMOR-BEARING ANIMALS

I. COMBINED IMMUNOCHEMICAL AND ENZYMIC DETERMINATION OF CATALASE IN LIVER CELL FRACTIONS

Abstract

The liver of a rat bearing ascites hepatoma cells (AH-49WH) was fractionated by a method modified after de Duve, and the catalase distribution was studied by both the enzymic and immunochemical determinations.
The values obtained by the immunochmical assay were in good agreement with those from the enzymic assay except in microsomes and supernatants, where the immunochemical result was higher than the enzymic one.
The data lead to the conclusion that the decrease of hepatic catalase activity in tumor-bearing rats mainly represents the decrease in the absolute concentration of enzyme protein probably caused by its impaired synthesis.