CATALASE OF TUMOR-BEARING ANIMALS

II. CHROMATOGRAPHIC ANALYSIS OF LIVER CATALASE

Abstract

The liver catalase of rats bearing ascites hepatoma (AH-49WH) was investigated by chromatography on DEAE-cellulose column. Four catalase fractions (I, II, III, and IV) were isolated by different concentrations of sodium chloride in eluting buffer solution, as is the case in the liver catalase of normal rats. Combined cell fractionation and chromatographic study have revealed that the Catalase-III in peroxisomes decreases most significantly in tumor-bearing rats while the Catalase-I and -II remain unchanged under the same conditions.