DEVELOPMENT OF VARIANT TUMOR CELLS OF YOSHIDA ASCITES SARCOMA PRODUCING α-FETOPROTEIN

Abstract

Three transplant lines of slow-growing variant cells producing α-fetoprotein, both in vivo and in vitro, were successfully obtained from a clonal cell population of the Yoshida ascites sarcoma cultivated in vitro for more than 5 months with or without mutagen treatment. Cells of these variants had altered karyotypes differing from each other and from the original Yoshida tumor cells. This finding may support the hypothesis on the hepatic cell origin of Yoshida ascites tumor.