臨床材料より最近分離した病原細菌のSulfamethoxazoleおよびTrimethoprim感受性と両剤の併用効果に関する試験管内実験
1973 年 21 巻 2 号 p. 110-116
詳細
1973 年 21 巻 2 号 p. 110-116
Agar dilution method was employed to determine antibacterial activity of sulfamethoxazole (SMX) and trimethoprim (TMP) against 53 strains of Staphylococcus aureus, 26 strains of Enterococcus, 36 strains of Escherichia coli, 33 strains of Klebsiella and 8 strains of Enterobacter which had been freshly isolated from clinical materials. Minimum inhibitory concentrations (MIC) of SMX were found to be within the range between 6.25 and >100 mcg/ml, while those of TMP were found to range from 0.05 to 1. 56mcg/ml, except for a single strain of Enterococcus and 2 Klebsiella strains.
The activity of SMX, TMP and their combinations were investigated employing various combinations of SMX and TMP at concentrations of 100 mcg/ml and their 2-fold diluted materials, respectively. Culture materials used were agar plates and liquid media. Combinations of SMX and TMP were found to have marked potentiating activity against microorganisms which were primarily sensitive to both SMX and TMP alone, i. e. the concentration of each drug contained in the combination was less than 1/4 the MIC of the corresponding drug when used alone.
However, when organisms were resistant to SMX with MIC higher than 100 mcg/ml, potentiation was found to be less remarkable.
