EFFECTS OF ANTIBIOTICS ON PHAGOCYTOSIS OF POLYMORPHONUCLEAR LEUKOCYTES TO PS. AERUGINOSA
1974 Volume 22 Issue 3 Pages 247-251
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1974 Volume 22 Issue 3 Pages 247-251
The effect of antibiotics on phagocytosis of Polymorphonuclear leukocytes (PMN) was studied by use of Ps. aeruginosa as the test organism. The authors reported in previous papers that strains of Ps. aeruginosa resistant to CB-PC differ from the strains sensitive to this antibiotic in the ratio of lipid contained in the cell surface layer, and that these two groups of strains differ in the sensitivity to Tris-EDTA-Lysozyme.
The present study clarified that these two groups of Ps. aeruginosa strains do not differ in resistance to phagocytosis of PMN. CB-PC, when added to a culture medium, increases phagocytosis of PMN on Ps. aeruginosa at a concentration as low as the 1/16 MIC. Meanwhile, GM gave no influence on the phagocytosis, and PL-B enhanced it only at concentrations as high as its MIC. Phagocytosis of PMN was not facilitated even when the cells of Ps. aeruginosa had been pretreated with any of these antibiotics. No relation was found out between phagocytosis and filament formation of the organism.
