抗生物質の腎毒性に関する研究 I

シロネズミ腎の蛋白代謝およびリソゾーム膜に及ぼす影響を中心に

抄録

In order to assess the potential nephrotoxicity exerted by various antibiotics with therapeutic dosis, effects of antibiotics administered into Wistar rat in vitro and in vivo were investigated in terms of protein synthesis, nucleic acid metablism and stability of lysosome.
Kanamycin, streptomycin, gentamicin, colistin as well as chloramphenicol inhibited the incorporation of orotic acid-6-¹⁴C into rat renal nuclear RNA.
The incorporation of orotic acid-6-¹⁴C into RNA of the free polysome fraction of the rat kidney slices was inhibited by kanamycin, streptomycin, colistin and tetracycline.
The incorporation of L-leucine-¹⁴C into the free polysome fraction was demonstrated to be reduced by streptomycin, chloramphenicol and tetracycline administered in vivo.
The incorporation of orotic acid-6-¹⁴C into RNA of the bound polysome fraction of rat kidney slices was reduced by kanamycin, streptomycin, chloramphenicol and cephalothin. The incorporation of L-leucine-¹⁴C into the bound polysomes fromes fraction was inhibited by streptomycin and colistin.
Marked reduction of the incorporation of L-leucine-¹⁴C into the free polysome fraction was noticed by kanamycin, streptomycin, gentamicin and chloramphenicol added directly to cell-free protein synthesis system. Also, marked reduction of the incorporation of L-leucine-¹⁴C into the bound polysome fraction was manifested by kanamycin, streptomycin, gentamicin and colistin.
Stability of lysosome was disturbed by either kanamycin or streptomycin.
Clinical implication of these nephrotoxicity of antibiotics was discussed.