Abstract
The subacute toxicity of T-1220 was tested using female beagle dogs treated with the daily intramuscular administration for a month, and compared with that of aminobenzylpenicillin (ABPC). T-1220 was injected to the dogs with daily doses of 250 and 1, 000 mg/kg, and ABPC was with daily dose of 1, 000 mg/kg. Control dogs were treated with physiological saline.
The results are as follows.
1) The signs showing severe pain were observed immediately after the administration in groups of T-1220 and ABPC, respectively, at 1, 000 mg/kg.
2) The bacterial flora of the bowel of the beagle dogs treated with T-1220 for a month was examined. The administration of T-1220 produced a slight decrease in total number of organisms that was mainly due to the disappearance of Bifidobacteria and Clostridia.
3) The GOT, GPT and LDH increased slightly in groups of T-1220 and ABPC at 1, 000 mg/kg. The histological examination, however, showed only hydropic changes of the hepatic cells and did not any inflammatory changes throughout the livers in both groups.
4) The group of T-1220 at 250 mg/kg had no abnormalities produced by the administration.
