STUDY OF BACTERIAL PROPAGATION IN THE MOUSE BODY AND IN MO ANTIBACTERIAL EFFECT OF TA-058 BY WHOLE BODY AUTOBACTERIOGRAPHY

Abstract

The autobacteriograms of mice infected intraperitoneally with E. coli KC-14 RFPr showed that the bacterial cells rapidly increased first in the peritoneal cavity and then in the blood. Fourteen hrs. after infection the cells were distributed in the whole body. On the contrary, in the mice infected intraperitoneally with P. aeruginosa No.12 a lag time of 8 hrs. elapsed before the bacterial cells started to increase in the peritoneal cavity. The cells were not distributed in the whole body even immediately before death. The bacterial number in the blood remained less than 10⁴cells/ml throughout.
The in vivo antibacterial effects of TA-058 and some reference drugs were compared in terms of bacterial growth on the autobacteriograms of mice infected with bacteria and treated with drug 1 hr. after infection. All the animals were sacrificed for autobacteriography 20-44 hrs. after infection shortly before the death of non-medicated, infected controls. The bacterial distribution and density in these mice were dependent on the administered dose of the antibiotics and their efficacy well reflected their ED₅₀. The excellent in vivo antibacterial effect of TA-058 was well substantiated in this autobacteriographical study.